Protein expression system is a system consisting of a host, an exogenous gene, a vector and auxiliary components, which enables the expression of exogenous genes in the host.
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Transient Expression Service for Mammalian Cells |
BEV-Insect Cell Protein Expression Service |
Yeast Protein Expression Service |
E. coli-Prokaryotic Protein Expression Service |
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Period: 4 - 6 weeks |
Period: 4 - 8 weeks |
Period:
6-10 weeks |
Period:
2 - 6 weeks |
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• Efficient expression vectors
• High
density cell culture
• Dedicated
leader sequences and connectors
• Optimally
screened mammalian cell expression system
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• Optimized expression vectors
• Efficient expression of intracellular and extracellular proteins
• Large-scale protein expression production capacity
• High-titer
virus packaging technology
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• Simple culture conditions • Multiple intracellular and extracellular expression schemes • Optimally screened high expression strains • Large-scale protein expression production capacity |
• Fast and economical • Multiple expression vectors and E. coli cells • Extensive experience in periplasmic space expression • Multiple protein purification and protein refolding techniques |
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Service steps |
Services |
Deliverables |
Delivery period |
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Vector construction |
(1) Gene synthesis and codon optimization (optional) (2) Fragment construction into an appropriate expression vector |
(1) Expression plasmid 1-5 ug
(2) Expression Plasmid Sequencing Report (3) Protein product of 0.1-5 mg, with a purity >85%. (4) Protein Product Report
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1 - 3 weeks |
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Cell transfection |
(1) Plasmid transfected cells (2) Optimization of fermentation conditions for expression test (3) Purification of laboratory-scale samples |
1 - 2 weeks |
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Amplification and fermentation |
(1) Amplification and fermentation (2) Protein purification (3) Protein Test Report |
1 - 2 weeks |
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Service steps |
Services |
Deliverables |
Delivery period |
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Vector construction |
(1) Gene synthesis and codon optimization (optional). (2) Fragment construction into the transfer vector. (3) Transfer vector recombination and recombinant bacmid extraction |
(1) Expression plasmid 1-5 ug (2) Expression Plasmid Sequencing Report (3) Recombinant virus (4) Protein product of 0.1-5 mg, with a purity >85%. (5) Protein Product Report
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2 - 3 weeks |
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Recombinant virus and amplification |
(1) Recombinant bacmid transfected cells (2) Recombinant virus amplification (3) Laboratory-scale fermentation and purification |
1 - 3 weeks |
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Amplification and fermentation |
(1) Amplification and fermentation (2) Protein purification (3) Protein Test Report |
1 - 2 weeks |
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Service steps |
Services |
Deliverables |
Delivery period |
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Vector construction |
(1) Gene synthesis and codon optimization (optional) (2) Fragment construction into an appropriate expression vector |
(1) Expression plasmid 1-5 ug (2) Expression Plasmid Sequencing Report (3) Positive Strain Screening Report (4) Optimal expression bacteria (5) Laboratory-scale expression results (6) Purification of 0.1-1 mg of target protein (7) COA of Protein Product
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1 - 3 weeks |
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Single colony screening |
(1) Vector linearization (2) Vector electrotransformation of Pichia pastoris cells (3) PCR screening of positive transformants |
1 - 3 weeks |
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Laboratory-scale expression |
(1) Four positive clones ed for expression Quantity of laboratory-scale validation (2) Expression analysis validation (WB) Optional: High-copy strain screening |
1 - 3 weeks |
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Amplification and fermentation |
(1) Amplification and fermentation (2) Protein purification (3) Protein Test Report |
2 - 4 weeks |
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Service steps |
Services |
Deliverables |
Delivery period |
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Vector construction |
(1) Gene synthesis and codon optimization (optional) (2) Fragment construction into an appropriate expression vector Note: Label-free, HIS label, sumo, GST, TRXA and MBP are optional |
(1) 1-5 ug liquid plasmid of expression vector plasmid (2) Protein product of 1-5 mg, with a purity >85% (3) Protein Product Report
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1 - 2 weeks |
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Expression test |
(1) Test expression of small volume fermentation (soluble/inclusion body expression) (2) Small volume purification test (3) Other options: label removal, inclusion body purification |
1 - 3 weeks |
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Delivery and reporting |
(1) Delivery of protein (2) Protein Test Report |
<1 week |
US: 3675 Market Street, Suite 200, Philadelphia, PA19104 Tel: +1 (215) 205-6963 | +086 027-65023363
E-mail: hui.wang@genevoyager.com
China: No128, Guanggu 7th Rd, East Lake High-tech Development Zone, Wuhan, China Tel: 17720522078
E-mail: marketing@genevoyager.com
US: 3675 Market Street, Suite 200, Philadelphia, PA19104 Tel: +1 (215) 205-6963 | +086 027-65023363
E-mail: hui.wang@genevoyager.com
China: No128, Guanggu 7th Rd, East Lake High-tech Development Zone, Wuhan, China Tel: 17720522078
E-mail: marketing@genevoyager.com
