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Vaccinia Virus (VV)

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Bovine pox virus is the largest and most complex DNA virus, with a linear double-stranded DNA genome of about 190 kb in length, encoding about 200 genes, with an envelope and brick-shaped viral particles of about 300x240x120 nm. The virus has a wide host range and can insert a large capacity of exogenous genes; the virus replicates and proliferates in the cytoplasm and does not integrate into the host cell genome. Currently, Western Reverse, Lister, Wyeth, Ankara, NewYork Vaccinia, Copenhagen and Titan strains are the most common strains, among which Western Reverse, Lister, Wyeth and Copenhagen strains have tumor lysis properties. The Western Reverse, Lister, Wyeth and Copenhagen strains were tumor soluble, and the Western Reverse strain had the strongest tumor solubility.

Structure of vaccinia virus (Harrison SC et al., Proc Natl Acad Sci U S A, 2004)

Service Content

Genevoyager can design and produce defective cowpox virus by knocking out specific genetic sites such as J2R (TK), A56R (hemagglutinin), F14.5, etc. for Western Reverse (WR) strains.  It is able to make it ineffective to replicate in normal cells and tumor-ive. Simultaneous insertion of exogenous genes, such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) can enhance the immune response. To further improve its tumor targeting and safety, poxvirus genetic modification can be performed according to customer requirements.

Customized Service

Customized product

Service content

Period (working day)

VACV (WR strain) (total volume 1.00E+9)

Conduct data research and design experimental protocols according to customer needs

5

plasmid construction

Conventional recombinant vectors

10

Insertion of the specified gene

10(Synthesis cycle is calculated separately

Virus recombination, spot picking, purification (one-step recombination)

12

Molecular identification

3

Recombinant virus amplification and concentration

5

Product Quality Control Report

5

Product Information

In-stock products

Product number

Contents

Fluorescent

Period

(working days)

Titer

(PFU/mL)

Total

volume PFU

Total volume PFU

VACV

(WR strain)

VV01001

VV-ΔTK+T7-RNA Polymerase

-

5-10

1.00E+9

1.00E+09

1.00E+10

VV01002

VV-ΔTK+EGFP

EGFP

VV03001

VV-ΔA56R-ΔTK

-

≥1.00E+9

1.00E+09

1.00E+10

VV03002

VV-ΔTK+EGFP-ΔI4L

EGFP

≥1.00E+9

1.00E+09

1.00E+10

Application Cases

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E-mail: hui.wang@genevoyager.com

China: No128, Guanggu 7th Rd, East Lake High-tech Development Zone, Wuhan, China Tel: 17720522078
E-mail: marketing@genevoyager.com

ABOUT US TECHNOLOGY PLATFORM

US: 3675 Market Street, Suite 200, Philadelphia, PA19104 Tel: +1 (215) 205-6963 | +086 027-65023363
E-mail: hui.wang@genevoyager.com

China: No128, Guanggu 7th Rd, East Lake High-tech Development Zone, Wuhan, China Tel: 17720522078
E-mail: marketing@genevoyager.com

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